Research
Activities


| Laboratori Ictiologia
Genètica, Departament de Biologia, Facultat de Ciències, Universitat de Girona, Spain. |
| Contact person (name and e-mail): |
| Persons working on brown trout
population genetics: |
| Nuria Sanz Ball-llosera,
Carles Pla-Zanuy, José Luis García-Marín |
| General research topics of the
laboratory: |
| Population structure,
Conservation and Management, Phylogeography. |
| General description of research on
brown trout population genetics: |
| A major research topic of
our laboratory is the conservation and management of native brown trout populations. In
particular, we study the population structure of Spanish brown trout populations, the
distribution of genetic variation within and between populations and the effects of
hatchery-outplants on those populations. Another important topic is the phylogeography of
brown trout since we depend on correct identification of the autochthonous form for the
study of interactions between introduced and native trout. Protein electrophoresis is
the major molecular tool employed in these studies. MtDNA D-LOOP sequencing has been
started recently. |

| List of projects (brown trout) for
the past 3 years: |
Study #1 |
| Researcher: |
Nuria Sanz (Ph.D student),
José Luis García-Marín, Carles Pla (supervisor) |
| Collaborators: |
Ana Almodovar |
| Source of project
funding: |
I.N.I.A. (Spanish National Institution
for Research on Agriculture). |
| Topic: |
Gene diversity, Stocking
effects, Stocking efficiency. |
| Objective: |
To study ecological and genetical
variables involved in population biology of brown trout in Central Spain. Identification
of past stocking activities. |
| Design: |
Streams in Central Spain are
genetically screened and ecological monitored during periods of two-three years. |
| Methodology: |
Protein electrophoresis,
mtDNA-RFLP. |
| Trout population: |
Resident population (& Hatchery
stock). |
| Origin of samples: |
Spain, South Atlantic (&
Atlantic Basin). |
| Status: |
Started in Jan. 1995, ongoing. |

Study #2 |
| Researcher: |
Nuria Sanz (PhD student),
José Luis García-Marín, Carles Pla (supervisor) |
| Source of project
funding: |
D.G.M.N.-D.A.R.P. (General Directorate
of Wild Life- Department of Agriculture, Fisheries and Livestocks, Autonomous Goverment of
Catalunya, Spain) & in house. |
| Topic: |
Gene diversity, Stocking effects,
Stocking efficiency. |
| Objective: |
To study genetic structure and
microdifferentiation among eastern Pyrenees trout populations. To assess genetic impact of
hatchery reared fish on native populations. |
| Design: |
Genetic survey of trout populations in
protected, fished and stocked areas. |
| Methodology: |
Protein electrophoresis |
| Trout population: |
Resident population
(& Hatchery stock). |
| Origin of samples: |
Spain (Mediterranean Sea Basin, Atlantic Basin). |
| Status: |
Started in 1996 as part of Miss Sanz
PhD thesis, ongoing. |

Publications (brown trout genetics)

Journal
papers:
- García-Marín J.L., Jorde P.E., Ryman N., Utter F. &
Pla C. 1991. Management implications of genetic differentiation between native and
hatchery populations of brown trout (Salmo trutta) in Spain. Aquaculture, 95,
235-249.
- Pla C. 1995. Ecological effects of genetically modified
fish: An overview. In: Pan-European conference on the potential long-term impact of
genetically modified organisms. pp. 217-222. Council of Europa Press, Strasbourg,
France.
- García-Marín J.L. & Pla C. 1996. Origins and
relationships of native populations of brown trout (Salmo trutta) in Spain. Heredity,
76, 313-323.
- García-Marín J.L., Sanz N. & Pla C. 1998. Proportions
of native and introduced brown trout in adjacent fished and unfished Spanish rivers. Conservation
Biology, 12, in press.
Technical Reports:
- García-Marín J.L. & Pla C. 1992. Les repeuplements de
truite commune dans les hauts bassins des rivières: éléments pour une politique de
gestion approprié. Journée Technique International (Oronoz) sur le Truite commune
pyrénéenne: Génétique et repeuplement. Recuil des interventions et conclusions: 65-81.
- García-Marín J.L. 1994. Diferenciación genética de la
trucha común (Salmo trutta) en España. Publicaciones de la UAB, 276 pp.
- Pla C. & García-Marín. 1995. Manual práctico para la
gestión sostenible de la pesca fluvial. Diego García de Jalón & Guido Schmidt
(eds.). pp. 169. AEMS. Girona, Spain.
Theses (eg, PhD and MSc theses):
- Jose Luis García-Marín. (1992). Diferenciación genética
de la trucha común, Salmo trutta, en España. Ph.D. Thesis. Universitat Autònoma
de Barcelona, Spain.
- Sanz, N. (1997). Anàlisi electroforètica de la truita
comuna, Salmo trutta, a la Conca alta de la Noguera Ribagorçana. M.Sc Thesis.
Universitat de Girona, Spain.

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