Dr Lisa Connolly
Lecturer in Food Toxin Safety
Postal Address:
Institute of Agri-Food & Land Use
Queen's University Belfast
David Keir Building
Stranmillis Road
Belfast BT9 5AG
Room: 01.320
Telephone: +44 (0)28 9097 6668
Fax: +44 (0)28 9097 6513
Email: l.connolly@qub.ac.uk
Administrative Responsibilities
- Module Co-ordinator 110ALU303 Food Quality & Safety
Further details on this module can be obtained by entering the specific code into the Book of Modules.
Research Profile
Lisa obtained her BSc in Biological Sciences at The University of Ulster in 1994. In 1999 she was awarded a PhD in Biological Sciences specialising in cancer research, multiple drug resistance and immunodiagnostics at Dublin City University. In 2000 Lisa took up a postdoctoral research position at the Veterinary Sciences Department of Queens University Belfast where she worked on a number of projects concerned with developing and applying new technologies for the detection of harmful contaminants in food. In 2006 she was appointed as Lecturer within The Institute of Agri-Food and Land Use where she is establishing a vital research programme focusing on endocrine disrupting environmental and food based toxins resulting from modern day industrial, agricultural or natural sources and the effects that these contaminants may have on human health.
Research statement
Lisa has developed major expertise in the discipline of binding protein technology encompassing both antibodies and cellular receptors in areas such as therapeutics, diagnostics and food safety. She has gained worldwide recognition and formal collaborative links in this area which has been the main focus of my research for more than fourteen years. In recent years I have focused on the implementation of binding proteins in food safety applications. New technologies must be developed to detect the broadening range of new and emerging chemical food contaminants created by modern day living. An understanding of the health risks we now face from these contaminants must also be fully investigated.
Indicators of Esteem
- Consultant in the area of Hybridoma Technology and Knowledge Transfer to Biotech companies (Fusion Antibodies Ltd, XenoSense Ltd) and public bodies (Agri-Food and Biosciences Institute) 2000 -
- Invited speaker, Department of Molecular Biology and Genetics, Liege University, Belgium, 2007
- Invited speaker at The National Institute for Cellular Biotechnology, Dublin City University, Ireland, 2007
- Member of the Committee of the Ulster Immunology Society 2006 -
Publications
Busk, O., Frizzell, C., Kalayou, S., Ndossi, D., Verhaegen, S., Uhlig, S., Connolly, L., Ropstad, E., Sorlie, M. (2011). Cytosol protein regulation in H295R steroidogenesis model induced by the Zearalenone metabolites α- and β-Zearalenol. Toxicon, In press.
Busk, O., Ndossi, D., Verhaegen, S., Connolly, L., Eriksen, G., Ropstad, E., Sorlie, M. (2011). Relative quantification of the proteomic changes associated with the mycotoxin Zearalenone in the H295R steroidogenesis model. Toxicon, 58: 533-542.
Plotan, M., Scippo, M.L., Muller, M., Antignac, J.P., Malone, E., Bovee, T., Mitchell, S., Elliott, C., Connolly, L., (2011). The application of reporter gene assays for the detection of endocrine disruptors in sport supplements. Analytica Chimica Acta, 700: 34-40.
Frizzell, C., Ndossi, D., Verhaegen, S., Dahl, E., Eriksen, G., Sorlie, M., Ropstad, E., Muller, M., Elliott, C.T., Connolly, L. (2011). Endocrine disrupting effects of Zearalenone, alpha- and beta-zearalenol at the level of nuclear receptor binding and steroidogenesis. Toxicology Letters, 206(2): 210-217.
Oplatowska, M., Connolly, L., Stevenson, P., Stead, S., Elliott, C. (2011). Development and validation of a fast monoclonal based disequilibrium enzyme-linked immunosorbent assay for the detection of triphenylmethane dyes and their metabolites in fish. Analytica Chimica Acta, 698: 51-6.
Connolly, L,, Ropstad, E., Verhaegen, S. (2011). In vitro bioassays for the study of endocrine disrupting food additives and contaminants. Trends in Analytical Chemistry, 30(2); 227-238.
Caim, K., Phillips, D,H,, Elliott, C.T., Van der Heidenm, E,, Scippom, M,L,, Muller, M,, Connolly, L., (2010). Removal of androgens and estrogens from water by reactive materials. Journal of Water Resource and Protection, 2: 990-993.
Chou, S., Haughey, S., Connolly, L., Del Castillo, M., Ames, J., Elliott, C. (2010). Immunochemical and Mass Spectrometric Analysis of Nε-(Carboxymethyl)lysine Content of AGE-BSA Systems Prepared With and Without Selected Antiglycation Agents. Journal of Agricultural and Food Chemistry, 58(22): 11955-11961.
Chou, S., Hull, G., Connolly, L., Elliott, C., Del Castillo, M.,, Ames, J. (2010). Effect of inhibitor compounds on N?-(carboxymethyl)lysine (CML) and Nε-(carboxyethyl)lysine (CEL) formation in model foods. Journal of Agricultural and Food Chemistry, 58(22): 12036-12041.
Connolly, L., Cai, K., Van der Heiden, E., Scippo, L., Muller, M., Tarbin, J., Elliott, C. (2009). Detection of glucocorticoid bioactivity in bovine urine samples using a reporter gene assay. Analytica Chimica Acta, 637(1-2): 321-327.
Stewart, L.D,, Hess, P., Connolly, L., Elliott, C.T. (2009). Development of a Pseudofunctional Biosensor Immunoassay for the Detection of the Okadaic Acid Group of Toxins. Analytical Chemistry, 81(24): 10208-10214.
Stewart, L., Elliott, C.T., Walker, A.D., Llamas, N., Curran, R., Connolly, L.. (2009). A novel monoclonal antibody to the marine biotoxin okadaic acid which also detects its analogues relative to their toxicity equivalency factors. Toxicon, 54: 491-498.
Walsh, N., Larkin, A., Kennedy, S., Connolly, L., Ballot, J., Ooi, W., Gullo, G., Crown, J., Clynes, M., O'Driscoll, L. (2009). Expression of Multi-drug resistance markers ABCB1 (MDR-1/P-gp) and ABCC1 (MRP-1) in renal cell carcinoma. BMC Urology, 9: 6. Doi: 10:1186/1471-2490-9-6.
Connolly, L., Thompson, C.S., Haughey, S.A., Traynor, I.M., Tittlemier, S., Elliott, C.T. (2007). The development of a multi-nitroimidazole residue analysis assay by optical biosensor via a proof of concept to develop and assess a prototype test kit. Analytica Chimica Acta, 598(1): 155-161.
Hagren, V., Connolly, L., Elliott, C.T., Lovgren, T., Tuomola, M. (2005). Rapid screening method for halofuginone residues in poultry eggs and liver using time-resolved fluorometry combined with the all-in-one dry chemistry assay concept. Analytica Chimica Acta, 529(1-2): 21-25.
O'Connor, R., Heenan, M., Connolly, L., Larkin, A., Clynes, M. (2004). Increased anti-tumour efficacy of doxorubicin when combined with sulindac in a xenograft model of an MRP-1-positive human lung cancer. Anticancer Res., 24(2A): 457-64.
Connolly, L., Fodey, T.L., Crooks, S.R.H., Elliott, C.T. (2003). The production and characterisation of an antibody to detect the coccidiostat toltrazuril and its metabolite ponazuril. Analyst, 128(5): 459-61.
Fodey, T.L., Connolly, L., Crooks, S.R.H., Delahaut, P., Elliott, C.T. (2003). Production and characterisation of polyclonal antibodies to a range of nitroimidazoles. Analytica Chimica Acta, 483(1-2): 193-200.
Connolly, L., Fodey, T.L., Crooks, S.R.H., Delahaut, P., Elliott, C.T. (2002). The production and characterisation of dinitrocarbanilide antibodies raised using antigen mimics. J. Immunol Methods, 264(1-2): 45-51.
Connolly, L., Moran, E., Larkin, A., Scheffer, G., Scheper, R., Sarkadi, B., Kool, M., Clynes, M. (2001). A new monoclonal antibody, P2A8(6), that specifically recognizes a novel epitope on the multidrug resistance-associated protein 1 (MRP1), but not on MRP2 nor MRP3. Hybridoma and Hybridomics, 20(5-6): 333-341.
Liang, Y., Meleady, P., Cleary, I., Connolly, L., Clynes, M. (2001). Selection with melphalan or paclitaxel (Taxol) yields variants with different patterns of multidrug resistance, integrin expression and in vitro invasiveness. European Journal of Cancer, 37(8): 1041-1052.
Larkin, A., Moran, E., Alexander, D., Doherty, G., Connolly, L., Kennedy, S.M., Clynes, M. (1999). A new monoclonal antibody that specifically recognizes the MDR-3-encoded gene product. International Journal of Cancer, 80(2): 265-271.
Clynes, M., Daly, C., NicAmhlaoibh, R., Cronin, D., Elliott, C., O'Connor, R., O'Doherty, T., Connolly, L., Howlett, A., Scanlon, K. (1998). Recent developments in drug resistance and apoptosis research. Critical Reviews in Oncology Hematology, 28(3): 181-205.
Heenan, M., O'Driscoll, L., Cleary, I., Connolly, L., Clynes, M. (1997). Isolation from a human MDR lung cell line of multiple clonal subpopulations which exhibit significantly different drug resistance. International Journal of Cancer, 71(5): 907-915.
Book chapters:
Connolly, L. (2009). Endocrine disrupting chemicals: origins, fates and transmission into the food chain in Endocrine disrupting chemicals in food. (Ed) Shaw I. Woodhead Publishing Ltd. (5) pp 103-125.
Verhaegen, S., Coyle, S., Connolly, L.M., O'Loughlin, C., and Clynes, M. (1998). Analysis of cell cycle and cell death mechanisms. In: Animal Cell Culture Techniques (M. Clynes, Ed.) Springer, Berlin.
