Dr Stewart Graham
Research Fellow
Postal Address:
Institute of Agri-Food & Land Use
Queen's University Belfast
David Keir Building
Stranmillis Road
Belfast BT9 5AG
Room: 01.303
Telephone: +44 (0)28 9097 6562
Fax: +44 (0)28 9097 6513
Email: stewart.graham@qub.ac.uk
Qualifications
BSc (Hons) Biochemistry, 2005; Queen's University, Belfast
MSc Biomolecular Structure and Function, 2006; Queen's University, Belfast
PhD, Metabolomics, 2009; Queen's University, Belfast
Research Profile
Stewart obtained his BSc (Hons) in Biochemistry in 2005 and his MSc in Biomolecular Structure and Function in 2006 from Queen's University, Belfast. Following this Stewart completed his PhD in the Institute of Agri-Food and Land Use (IAFLU) on NMR metabolomics. From 2009 to present day Stewart has been employed by AFLU and is currently completing his second post-doctoral position. To date Stewart has been involved in the preparation of commercial ELISA kits to detect antibiotics used as growth promoters in chicken production (BioCop Project) and model building using 1H NMR spectral data from bovine plasma samples treated with growth promoting hormones to predict abuse in cattle production (ASSET Project). In addition, Stewart has successfully utilised 1H and 2-dimensional NMR in various metabolomic investigations, previously and in conjunction with GC-MS and HPLC and has used NMR to profile beef samples aged over specific time periods. Stewart has also been utilising 1H and 2-dimensional NMR to analyse soil samples taken from a variety of sites across the island of Ireland to determine if geographical location has any impact on the polar content of the samples. In addition he has employed 1H and 2-dimensional NMR to analyse brain samples from mice with Alzheimer's disease to determine if a model can be built to aid in early detection of the disease. More recently Stewart has been using gas chromatography coupled with mass spectrometry to analyse the fatty acid methyl ester content of plasma taken from animals infected with bovine tuberculosis and control animals to determine if a lipidomic approach can be used to differentiate between the two and to reveal any as yet unrecorded perturbations due to the disease. Currently Stewart is utilising Near Infrared and Raman spectroscopy to profile grain and feed samples as a quick means of quality control in the agricultural sector.
Publications
Graham, S.F., Chevallier, O., Roberts, D., Elliott, C. and Green, B. (2012). A High Resolution Mass Spectrometry approach for studying Brain Metabolite changes in Alzheimer's Disease. Submitted to Proceedings to the National Academy of Sciences.
Graham, S.F., Holscher, C., McClean, P., Elliott, C. and Green, B. (2012). Brain tissue and plasma used to metabolically phenotype Alzheimer's disease in APP/PS1 mice. In preparation to be submitted to the Journal of Biological Chemistry.
Graham, S.F., Haughey, S.A., Ervin, R.M., Cancouet, E., Bell, S. and Elliott, C.T. (2011). The Application of Near-Infrared Transmittance (NIR) and Raman Spectroscopy to detect adulteration of oil used in animal feed production. Food Chemistry, DOI:10.1016/j.foodchem.2011.11.136.
Graham, S.F., Ruiz-Aracama, A., Biolatti, B., Elliott, C. and Mooney, M. (2012). Use of 1H NMR Metabolomic Plasma Profiling Methodologies to Identify Animals Treated with Growth Promoting Agents. Analytical and Bioanalytical Chemistry, DOI: 10.1007/s00216-012-5815-z.
Haughey., S., Graham, S.F., Cancouet, E. and Elliott, C. (2012). Near-Infrared Reflectance Spectroscopy (NIRS) to Detect Melamine Adulteration of Soya Bean Meal. Food Chemistry, DOI: 10.1016/j.foodchem.2012.01.068.
Graham, S.F., Farrell, D., Kennedy, T., Farmer, L., Elliott, C. and Moss, B. (2012). A comparative study using 1H NMR and HPLC biochemical profiling of longissimus dorsi beef tissue to investigate the effect of hanging and ageing on amino acid concentrations. Food Chemistry, 10.1016/j.foodchem.2012.03.047.
Graham, S.F., Kennedy, T., Farmer, L. and Moss, B. (2010). The application of NMR to study changes in polar metabolite concentrations in beef longissimus dorsi stored for different periods post mortem. Metabolomics, 6(3): 395-404.
Graham, S.F., Hollis, J., Migaud, M. and Browne, R.A. (2009). Analysis of betaine and choline contents of aleurone, bran and flour fractions of wheat (T. aestivum L.) using 1H NMR spectroscopy. Journal of Agricultural and Food Chemistry, 57(5): 1948-1951.
Graham, S.F., Amigues, E., Migaud, M. and Browne, R.A. (2009). The application of NMR based metabolomics for mapping metabolite variation in European Wheat. Metabolomics, 5: 302-306.
Graham, S.F. and Browne, R.A. (2009). Anther extrusion and Fusarium head blight resistance in European wheat. Journal of Phytopathology, 157: 580-582(3).
Conference Presentations
Graham, S.F., Kennedy, J.T., Gordon, A.W., Farmer, L.J., Elliot, C.T. and Moss, B.W. (2010). Potential of nuclear magnetic resonance spectroscopy to measure biochemical changes during post mortem aging of beef hung by Achilles or pelvic suspension. Advances in Animal Science, Proceedings of the British Society of Animal Science, Belfast, pp. 125, April 2010.
Mooney, M., McGrath, T., Graham, S.F. and Elliott, C. Effect-based approaches to detect illegal growth promoter use in food producing animals. 6th International Symposium on Hormone and Veterinary Drug Residues Analysis, Ghent, 1st-4th June 2010, pp. 25. Belgium, University Forum.
Conference Poster Presentations
Graham, S.F., Farrell, D., Kennedy, J.T., Gordon, A.W., Farmer, L.J., Elliot, C.T. and Moss, B.W. Comparing GC-MS, HPLC and 1H NMR analysis of beef longissmus dorsi tissue extracts to determine the effect of suspension technique and ageing. Food Integrity and Traceability Conference, Queen's University, Belfast, 21st-24th March 2011.
Graham, S.F., Amigues, E., Migaud, M. and Browne, R.A. NMR metabolomics for mapping metabolite variation in European wheat. Presented at the 10th International Fusarium Workshop and Fusarium Genomics Workshop, Sardinia, Italy, 30th August-2nd September 2008.
