Experimental Biochemistry

Overview

Introduction to the Biochemistry Laboratory, General Laboratory Procedures, Chromatography Techniques, Electrophoresis, Centrifugation in Biochemical Research
Radioisotopes in Biochemical Research, UV/vis, fluorescence and CD spectroscopy, Mass Spectrometry, X-ray crystallography, Protein crystallisation, Biochemical thermodynamics, Biomolecular Interactions, Enzyme Kinetics, Immunological techniques, and Application of computers in biochemistry.

Learning Objectives

Upon successful completion of this module students will be able to:

LO1: Describe and explain the principles and theory of key experimental techniques used in biochemistry
LO2: Develop key practical skills in specific analytical techniques.
LO3: Apply a broad knowledge of theoretical and practical concepts to evaluate the significance of experimental/scientific data.
LO4: Develop problem solving skills.
LO5: Develop communication skills including practical report writing.
LO6: Develop and understanding of Good Laboratory Practice (GLP)

Skills

Competence in the laboratory, including the ability to plan and execute an experiment and to evaluate and interpret the resulting data. Competence in critically evaluating experimental data. Awareness of the importance of working safely in the laboratory in accord with Health and Safety protocols and good working practices.

Assessment