Instrumental Methods of Analysis

Overview

Summary of Lecture Content:
 Introduction to separations and chromatography
 Liquid Chromatography
 Gas Chromatography
 Infrared Spectroscopy
 Nuclear Magnetic Resonance
 Mass spectrometry
 Hyphenated analytical techniques

Summary of Workshop Content:
 HPLC instrumentation
 HPLC method development
 NMR spectroscopy
 Mass spectrometry

Learning Objectives

On completion of this module students should be able to:
 Understand the principles, instrumentation, and applications of chromatography, spectroscopy,
and mass spectrometry.
 Explain the fundamentals of Liquid Chromatography (LC) and Gas Chromatography (GC),
including key factors affecting separation efficiency.
 Interpret chromatographic, IR, NMR, and MS data to identify compounds and assess analytical
performance.
 Compare the strengths, limitations, and applications of different instrumental techniques.
 Understand the advantages of hyphenated techniques (e.g., LC-MS, GC-MS).

Skills

Students are expected to demonstrate the following skills on completion of the module:
 Explain the principles and applications of key analytical techniques (LC, GC, NMR, IR, MS).
 Interpret chromatographic, IR, NMR, and MS data to identify compounds.
 Troubleshoot common issues in chromatography and spectroscopy.
 Evaluate analytical data for accuracy, precision, and reliability.
 Use software tools for spectral analysis and data interpretation.
 Communicate findings clearly in a scientific format.

Assessment