"Protic Ionic Liquids: Solvent Properties and Application to Proteins"
Protic ionic liquids (PILs) are cost efficient “designer” solvents which can be tailored to have properties suitable for a broad range of applications1-2. More recently ionic liquids (ILs) are being combined with molecular solvents, and these binary or multicomponent IL solvents enables more control over the solvent environment, and typically reduces the cost and viscosity.
However, there are relatively few structure-property studies which look at these more complex mixtures. We have explored the solvation properties of common PIL-molecular solvents using various techniques, including Kamlet-Aboud-Taft parameters determined from solvatochromic dyes, and MD simulations3-4. These have identified many interesting solvent properties of these solutions and gives insight into their interactions with solutes. This lead to the use of PIL-water solvents having the potential to control protein solubility and stability, which in turn are critical for applications in bioprocessing, protein crystallography and cryopreservation. In this presentation I will cover some solvation properties of PIL solvents, along with the protein structure changes of proteins in IL solvents which we have explored using spectroscopic and advanced small angle x-ray scattering (SAXS) methods5.
1. Greaves, T. L.; Drummond, C. J., Protic ionic liquids: properties and applications. Chem. Rev. 2008, 108 (1), 206-237.
2. Greaves, T. L.; Drummond, C. J., Protic ionic liquids: evolving structure−property relationships and expanding applications. Chem. Rev. 2015, 115, 11379-11448.
3. Yalcin, D.; Christofferson, A. J.; Drummond, C. J.; Greaves, T. L., Solvation properties of protic ionic liquid – molecular solvent mixtures. Phys. Chem. Chem. Phys. 2020, 22, 10995-11011.
4. Yalcin, D.; Drummond, C. J.; Greaves, T. L., Solvation properties of protic ionic liquids and molecular solvents. Phys. Chem. Chem. Phys. 2020, 22, 114-128.
5. Han, Q.; Smith, K. M.; Darmanin, C.; Ryan, T. M.; Drummond, C. J.; Greaves, T. L., Lysozyme conformational changes with ionic liquids: Spectroscopic, small angle x-ray scattering and crystallographic study. Journal of Colloid & Interface Science 2020, DOI: 10.1016/j.jcis.2020.10.024.