An MRC-funded postdoctoral position is available to work on a project entitled: Investigating the role of mitochondrial Complex I in cellular response to hypoxia.
Mitochondrial Complex I plays a critical role in the regulation of energy generation in the cell. The activity of this enzyme can be modulated by oxygen availability thereby mediating ischaemia/reperfusion injury. This project aims to unravel the role of mitochondrial Complex I in hypoxia and experiments will be conducted on preparations of isolated mitochondria and cultured cells. Techniques which will be utilised during the project include: isolation and functional assays on mitochondria and cells; enzyme kinetics; basic molecular biology; cell culture; fluorescence imaging techniques.
Highly qualified and motivated candidates with a PhD or in process of submitting their thesis in a relevant subject area (Biochemistry / Cell biology / Molecular biology) are encouraged to submit their application. Preference will be given to candidates with a proven track record in bioenergetics/mitochondrial physiology and enzymology. Experience in in vivo imaging and immunochemistry techniques is desirable. Proficiency in English and excellent communication and social skills are required.
Galkin, A., Abramov, A., Frakich, N., Duchen, M., Moncada, S. (2009) Lack of oxygen deactivates mitochondrial complex I: Implications for ischemic injury? J.Biol.Chem., 284: 36055-36061. PMID: 19861410.
Galkin, A., Meyer, B., Wittig, I., Karas, M., Schägger H., Vinogradov, A., Brandt, U. (2008). Identification of the mitochondrial complex I ND3 subunit as a structural component involved in the active/de-active enzyme transition. J.Biol.Chem., 283: 20907-20913. PMID: 18502755.
Galkin, A. and Moncada, S. (2007). Nitrosation of mitochondrial complex I. J.Biol.Chem., 282: 37448-37453. PMID: 17956863.
Project on mitochondrial Complex I studies is available for individuals with self arranged funding. Enquiries from motivated post graduates/post docs who have independent funding or fellowships, and who have a desire to work at the forefront of mitochondrial physiology are always welcome.